唑啉头孢菌素中间体(裂解物)的高效液相色谱分析

ANALYSIS OF INTERMEDIATE OF CEFAZOLIN (Cleavage Product) BY HPLC

摘要: 本文采用YWG-NH_2色谱柱,以0.6M醋酸盐缓冲溶液为洗脱液,于紫外检测波长272nm处,用外标法或追加法,对唑啉头孢菌素中间体7-氨基-3-[2-(5-甲基-1,3,4-噻二唑)-硫甲基]-3-头孢-4-羧酸(简称裂解物)进行定量分析,两种方法的相对平均偏差为3％。方法可供生产过程中进行质量控制之用。
- 高效液相色谱法 /
- 唑啉头孢菌素
Abstract: High performance liquid chromatography was employed for the quantitative determination of intermediate of cefazolin, 7-amino-3-(2-(5-methyl-1, 3, 4, thiadiazolyl) thiomethyl)- △3 cephem-4-carboxylic acid which is so called as "cleavage product". Good assay result can be obtained by using external standardization or standard addition method, on YWG-NH2 column with 0.6M acetate buffer solution as mobil phase and UV detector (at 272nrn). The average relative deviation of these two methods is 3%. The proposed method is suitable for the quality control in the process of manufacture of cefazolin.
- HPLC /
- Cefazolin