薄层扫描测定溴丁东茛菪碱中氢溴酸东莨菪碱的含量

TLCS DETERMINATION OF SCOPOLAMINE HYDROBROMIDE IN SCOPOLAMINE BUTYLBROMIDE

摘要: 本文用硅胶 G 薄层板,以醋酸乙酯-丙酮-25.0～28.0%氨水(60∶20∶1)为展开剂,分离溴丁东茛菪碱及氢溴酸东莨菪碱;用改良的 Dragendofff 试剂作显色剂,测得氢溴酸东莨菪碱的 R_f 值为0.34、溴丁东莨菪碱在原点不移动;用外标法双波长薄层扫描(测定波长495nm,参比波长750nm)测定氢溴酸东莨菪碱的含量。回收率为100.1%(n=6),变异系数为3.5%。

Abstract: Silica gel G TLC with ethyl acetate-acetone-25-28% ammonia water(60:20:1) as developing solvent was usd to separate scopolamine hydrobromide from scopolamine butylbromide. Spots were visualized with modified Dragendorff reagent befor using dual wavelength TLCS to determine the content of scopolamine hydrobromide. The average recovery was found to be 100.1%(n=6) with coefficient of variation being 3.5%.