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三波长分光光度法中用计算机选择三波长组合的方法

SELECTION OF OPTIMAL GROUPS OF THREE WAVELENGTH WITH MICROCOMPUTER

  • 摘要: 应用计算机选择三波长组合须先在 UV-250(或 UV-300)分光光度计上打印出待测组份和干扰组份各自的吸收值,然后在 TRS-80微处理机上用“UV-TwS.1”程序完成挑选最佳三波长组合的工作,输出前10组|△A|最大的三波长组合。根据挑选波长组合在751G 分光光度计上精选确定后再进行测定。所得工作曲线及回收率数据用“UV—TWC、2”程序进行处理。对盐酸氯丙嗪注射液,按前述步骤在手动式(751G)分光光度计上用三波长法进行了测定,平均回收率为100.3±0.28%。五批结果均符合中国药典的要求,标准差在1%以内。文中还对从计算机上选出的126个组合中,选取的不同|△A|值的5组波长组合进行了工作曲线和回收率的测定比较。

     

    Abstract: The following steps are taken in selecting the groups of three wavelength with microcomputer: First, absorbances of the constituent under test and interfering substance are determined and printed by using UV-250(or UV-300) When UV—751G is used, a proper wavelength range should be chosen. Then, selecting optimal groups of three—wavelength is performed through the program "UV—TWSI" with microcomputer TRS-8O and the 10 groups, with maximal absolute values of △A are printed. Finally, all the calculations are conducted through the program UV-TWC2. Analysis of chlorpromazine hydrochloride injection is taken as an example. According to steps described-above, the manual spectrophotometer ( UV—751G) is used to determine five batches of samples the average recovery is found to be 100.3±0.28%, and the accuracy and precision of the results are found to meet the analytical requirements of the pharmaceutical preparations, the relative standard deviation is in the range of 1%.

     

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