应用改进的GLC法研究扑尔敏控释微丸的体内外相关性

AN IN VITRO AND IN VIVO CORRELATIVITY STUDY OF CHLORPHENIRAMINE CONTROLLEDRELEASE PELLETS BY MODIFIED GLC-ASSAY

摘要: 本文改进了扑尔敏血药浓度的气相测定法。选用安定为内标,在异辛烷一次提取全血后用N~(63)电子捕获器检测。结果表明,在0.5～12ng/ml范围内,扑尔敏及安定的色谱峰面积之比(Ac/Ad)与血液中两者浓度之比(Cc Cd)有良好的线性关系(r=0.996),回收率在90％以上(平均偏差4％)。应用此法测定了人口服扑尔敏微丸及速释微丸的血药浓度,并由此计算得出控释微丸的体内吸收百分数(Pi)与体外溶出百分数(Po)间的相关方程式为Po=0.9597Pi 3.3225(r=0.916)

Abstract: A determination of chlorpheniramine in human blood by GLC-assay, using an electron capture detector was described. Diazepam was used as the internal standard. The method was used in study on the correlativity between in viyo absorption and in vitro dissol