重组菌包含体中白细胞介素—2的分离

Study on the Preparation and Their Propertives of Recombinant Interleukin-2 Inclusion Bodies

摘要: 白细胞介素-2(IL-2)是调节免疫功能的淋巴因子。重组白细胞介素-2(rIL-2)的表达成功。为IL-2的基础研究和临床应用提供了条件。由于rIL-2在细菌中的高效表达,其形成包含体存在细菌胞浆中,难以溶解,给纯化工作带

Abstract: Recombinant interleukin-2 (rIL-2) expressed by E. Coli appears in the form of undissolvable and polymeric inclusion bodies in the E. Coli cytoplasm. During the process of purification, the purity of rIL-2 existing in inclusion bodies was raised by dissolv