化学－酶法合成甜味天苯二肽

Chemico-Enzymatic Synthesis of Sweetener Aspartame

摘要: 为探索天－苯甜二肽生产新技术，以马来酐与Ｌ－苯丙氨酸甲酯为原料经化学法缩合成马来酰－Ｌ－丙氨酸甲酯，并将后者异构化成富马酰－Ｌ－苯丙氨酸甲酯（ＦＰＭ），收率９５％；再以ＦＰＭ及氨或氨供体为底物，经Ｅ．ｃｏｌｉＣＰＵ８９０１转化为天－苯甜二肽，产物浓度为５．６ｍｇ／ｍｌ。

Abstract: This paper deals with the chemico-enzymatic synthesis of sweetener aspartame. N-Maleylphenylalanine methyl ester was prepared by treating maleic anhydrid with L-phenylalanine methyl ester and isomerized to N-fumarylphenylalanine methyl ester (FPM),yield 9