一种新酶放大系统及其应用
A New Enzyme Amplification System and Its Application
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摘要: 建立了一种能广泛用于酶标免疫吸附分析的新酶放大系统。该系统采用Ｌ－乳酸脱氢酶、乳酸氧化酶、辣根过氧化物酶、ＮＡＤＨ和３，３＇，５，５＇－四甲基联苯胺组成一个循环体系，用以放大碱性磷酸酶产生的信号。与其它酶放大系统相比，具有灵敏度高和成本低的特点。Abstract: A new enzyme amplification system which can be widely used in ELISA was developed.In this system,a complex of L-lactic dehydrogenase,lactate oxidase,horseradish peroxidase,NADH and 3,3',5,5'-tetramethylbenzidine were used to compose a cycling system to in
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