HPLC β-环糊精手性流动相添加剂的研究Ⅰ.β-环糊精及其甲基衍生物在 ODS固定相上的吸附

The Investigations into β-Cyclodextrinas HPLC Mobile Phase Chiral Additives　I.The Adsorption of β-Cyclodextrin and Its Methylated Derivative on an ODS Stationary Phase

摘要: 通过ＨＰＬＣ结合旋光光度定量的方法，探讨了流动相添加剂β－环糊精及其２，６－二甲基衍生物的吸附性质。结果表明β－环糊精在ＯＤＳ固定相上不吸附，而其２，６－二甲基衍生物则呈典型的单分子层吸附。

Abstract: The adsorption characteristics of β-CD and its 2, 6-dimethyl derivative as mobile phase additives were examined by HPLC chromato graphic methods associated with a polarimetric method for quantification. Results showed that the β-CD does not adsorbe onto the ODS stationary phase but its 2, 6-dimethyl derivative produces a typical Langmuir′s isotherms of a monolayer adsorption.