Abstract: The effect of 3,5-diisopropylsalicylic acid(3,5-DIPS) and its copper complex tetrakis-μ-3, 5-diisopropylsalicyla todiaquo dicopper(Ⅱ)Cu(Ⅱ)2(3,5-DIPS)4(H2O)2 on the status ofglutathione(GSH) in a queous solution and in the serum fraction of the human blood has been studied using spectrophotometric method. Glutathione in aqueous solution was oxidized in the presence of both (3,5-DIPS) and Cu(Ⅱ)2(3,5-DIPS)4(H2O)2 due to the air oxidation o f GSH to GSSG. The addition of 3,5-DIPS to the serum fraction of the human blood caused leakage of GSH, more likely from the albumin, giving higher GSH levels than control. Cu(Ⅱ)2(3,5-DIPS)4(H2O)2 increased the the serum GSH levels more effectively and in dose dependent manners. This observationisin agreement with the previous reports that copper complexes of chemicals and/or drugs are more effective in treating various diseases and are better active scavangers offree radicals than the parent chemicals and/or drugs.