Abstract: Active antibody membrane on piezoelectric quartz crystal was prepared with the mixture of 5% polyvinyl alcohol(PVA) and 10 u/ml insulin antibody. The results measured by radioimmunoassay and acoustic immunosensor show that there are better functions in the strength, responsibility, and penetration of the membranes . The binding amount of insulin to the certain concentration of antibody immobilized in the membrane is better correlative to the concentration of insulin in samples.