均匀设计和球面对称设计在螺旋藻多糖最佳工艺研究中的应用比较

Comparative of Uniform Design with Spherical Symmetric Design in the Study of Extraction Conditions for the Polysaccharide from Spirulina platensis (SP)

摘要: 通过均匀设计和球面对称设计方法考察螺旋藻多糖提取过程中,提取温度、提取时间、提取溶液的pH三个因素对多糖分离的影响,以期获得最佳的提取工艺。通过球面对称设计回归方程得出的优化条件是:提取温度96.42C,提取时间7.77h.溶液pH8.24,多糖得率为5.13％;通过均匀设计得出的优化条件是提取温度77.3C,时间6.99h,溶液pH10.85,多糖得率为4.21％。球面对称设计优化条件的可信度高于均匀设计。

Abstract: This paper deals with the use of uniform design and spherical symmetric in the study of optimum extraction conditions for the polysaccharide from Hpirulina piatensis (SP). The result, showed that in the uniform, and spherical symmetric design, the optimum