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去乙酰基壳聚糖在亲和层析纯化胰蛋白酶中的应用

Application of Chitosan in Purifying Trypsin with Affinity Chromatography

  • 摘要: 首次用环氧氯丙烷活化的去乙酰基壳聚糖(Chitosan)为载体,鸡卵粘蛋白(Ovomu-coid,简称CHOM)为配基,制备了胰蛋白酶(Trypsin)的亲和吸附剂,纯化效率与琼脂糖(Sepharose-4B)相近,为47倍,纯化的Trypsin经SDS-PAGE电泳为单一区带。表明Chitosan可以代替Sepharose-4B作为亲和层析的载体。

     

    Abstract: With Chitosan activated by epichlorohydrin as carrier and with ovomucoid as ligand the affinity adsorbent for purification of trypsin was prepared for the first time The efficiency of this adsorbent in purifying Trypsin was close to Sepharose 4B affini

     

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