人白细胞介素6表达的核因子蛋白在大肠杆菌中的表达与鉴定

Recombinant Expression of Human NF IL6 in E coli and Its Bioactivity Determination

摘要: 将ＮＦ－ＩＬ６基因与谷胱甘肽转移酶（ＧＳＴ）基因融合并成功地表达出具有生物活性的ＮＦ－ＩＬ６，经ＧｅｌＲｅｔａｒｄａｔｉｏｎ分析证实该重组蛋白能与其ＤＮＡ识别元件结合。实验还发现，虽然重组蛋白的表达率较低，但能形成不溶性包含体

Abstract: NF IL6, a nuclear factor for interleukine 6 expression, is a member of C/EBP family It plays an important role in gene regulation of inflammation, differenciation, acute phase reaction, forming of cytokine net, tumoregenesis and apoptosis In this r