衍生化寡糖的HPLC/ESI-MS分离和序列分析

HPLC/ESI-MS Analysis of Oligosaccharides as 1-Phenyl-3-methyl-5-pyrazolone Derivatives

摘要: 采用麦芽糖、麦芽三糖至麦芽七糖作为标准化合物，1－苯基－3－甲基－5－吡咯唑酮衍生化，HPLC／ESI－MS分离及结构分析。结果表明反相液相色谱能将该寡糖衍生物较好地分离；ESI响应强，准分子离子峰为基峰；源内碰撞诱导解离质谱图上有寡糖的序列信息。实验为进一步分析生物体内的寡糖提供了研究方法。
- 寡糖 /
- 序列分析 /
- 衍生化 /
- 1-苯基-3-甲基-5-吡咯唑酮 /
- 高效液相色谱/电喷雾离子化质谱法
Abstract: The 1-phenyl- 3- methyl- 5- pyrazolone derivatives of reducing oligosaccharides, maltose to maltoheptaose, were investigated using HPLC/ESLMS. The derivatives could be well separated in ODS column. It also gave good response signals in the online electros
- Oligosaccharide /
- Sequential analysis /
- Derivatization /
- 1-Phenyl-3-methyl-5-pyrazolone /
- HPLC/ESIMS