酶法合成L—丝氨酸及反应液中氨基酸的分离

Enzymatic Production of L Serine and Separation of Amino Acids from Reaction Solution

摘要: 含ｇｌｙＡ的基因工程菌所表达的ＳＨＭＴ可催化甲醛和甘氨酸特异地合成Ｌ－丝氨酸。对酶法合成的部分条件进行了优化，并根据甲醛滴定的原理，确定了通过检测ｐＨ变化控制甲醛流加速度的方案，最终的反应液中Ｌ－丝氨酸浓度达０．２ｍｏｌ／Ｌ。反应结束后的酶反应液中同时含有甘氨酸和丝氨酸，利用国产７１７树脂获得较为理想的分离效果，丝氨酸的收率达７７％，高效液相分析表明其中不含甘氨酸等杂质。

Abstract: Serine hydromethyltransferase (SHMT) expressed by genetic engineered bacteria can synthesize L serine from HCHO and glycine. Some reaction conditions were optimized. Based on the principle of HCHO titration, the feed rate of HCHO solution was controlled