聚乙二醇对大肠杆菌L—天冬酰胺酶的化学修饰

Chemical Modification of Escherichia Coli L-Asparaginase with Polyethylene Glycol

摘要: 用琥珀酸酐及琥珀酰亚胺两步活化的单甲氧基聚乙二醇对大肠杆菌Ｌ－天冬酰胺酶进行化学修饰、修饰反应的酶活回收率保持在４０％以上，修饰酶的抗热，抗胰蛋白酶水解能力有所提高，抗原性显著下降。
- 天冬酰胺酶 /
- 化学修饰 /
- 单甲氧基聚乙二醇 /
- 抗肿瘤
Abstract: E.coli L asparaginase was modified with SS PEG [methoxypolyethylene glycolyl succinimidyl succinate] which was prepared in two steps.The modified enzyme activity was maintained 44.52% while the antigenicity was greatly reduced.The modified L aspa
- L-asparaginase /
- Monomethoxypolyethylene glycol /
- Chemical modification