聚乙二醇对大肠杆菌L—天冬酰胺酶的化学修饰

Chemical Modification of Escherichia Coli L-Asparaginase with Polyethylene Glycol

摘要: 用琥珀酸酐及琥珀酰亚胺两步活化的单甲氧基聚乙二醇对大肠杆菌Ｌ－天冬酰胺酶进行化学修饰、修饰反应的酶活回收率保持在４０％以上，修饰酶的抗热，抗胰蛋白酶水解能力有所提高，抗原性显著下降。

Abstract: E.coli L asparaginase was modified with SS PEG methoxypolyethylene glycolyl succinimidyl succinate which was prepared in two steps.The modified enzyme activity was maintained 44.52% while the antigenicity was greatly reduced.The modified L aspa