原生质体紫外诱变技术筛选红霉素高产菌株的研究

Screening High-yield Strains of Streptomyces erythreus by UV Mutagenesis

摘要: 将红霉素产生菌－红色链霉菌的菌丝体经溶菌酶２壁后获得的原生质体，用紫外线照射一定时间后，在再生培养基上得到再生菌株，通过活性测定，挑选抑菌圈大而清晰的再生菌株进行摇瓶筛选，最后得到比对照菌株效价提高２２５．８％的高产菌株ＨＰ－３。

Abstract: Using lysozyme action on the mycelia, the Streptomyces erythreus protoplasts were obtained, then treated by UV light for certain time, the mutated strains were selected by potency determination after shake flasks fermentation, and was obtained a high y