人绒毛膜促性腺激素新的制备方法的研究

A New Preparation Method of Human Chorionic Gonadotropin

摘要: 目的：提高人绒毛膜促性腺激素（HCG）的活性回收率，简化HCG的纯化步骤。方法：pH4.0,0.05mol/LHOAc-NaAc缓冲液提取，人造沸石吸附和DEAE－纤维素层析法。结果：该法可将HCG粗品（12IU／mg)纯化到3200－3600IU／mg，活性回收率在70％至80％之间。

Abstract: AIM To improve the purification method of Human Chorionic Gonadotropin (HCG). METHODS Extracted with 0.05 mol/L HOAc NaAc buffer (pH 4.0) and then purified by permutit absorption or chromatography on DEAE cellulose. RESULTS and CONCLUSION HCG can pu