联苯胺荧光分光光度法测定一氧化氮合酶活力

Establishment of Benzidine-fluorescence Spectrophotometry to Determine Nitric Oxide Synthase Activity in Biological Sample

摘要: 目的：建立联苯胺荧光分光光度法测定一氧化氮合酶（NOS）活力的方法。方法：采用联苯胺荧光分光光度法测定生物样品中NOS在单位时间内生成的一氧化氮（NO）量来计算NOS活力。结果：联苯胺荧光分光光度法测出的NOS活力与文献报道基本一致。结论：联苯胺荧光分光光度法具有简便，迅速和重现性好的,适合于大批量组织器官样品中的NOS活力测定。

Abstract: AIM An assay method for nitric oxide synthase(NOS) activity was developed based on fluorometric spectrophotometry with benzidine. METHOD Formation of NO in unit time was measured by benzidine fluorescence spectrophotometry, which was used to determine