石斛及其常见混淆品的matK基因序列比较
A Comparison of matK Sequences between Herba Dendrobii (Shihu) and Its Adulterant Species
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摘要: 目的：比较几种药典收载石斛与常见市场混淆品种的matK基因序列，为石斛类药材的鉴定提供分子依据。方法：PCR扩增、测序、PAUP软件分析。结果：非石斛属的几种混淆品与正品石斛间的matK基因序列差异远大于正品石斛间的差异，PAUP 4．0构建的树型图亦显示几种石斛属植物聚在一起，而非石斛属植物聚在外方。结论：通过matK基因序列可以分析石斛及其混淆品间的遗传关系，将正品与混淆品区别开来。Abstract: AIM\ To authenticate Herba Dendrobii from its adulerant species by matK sequences. METHOD\ The double strand DNA were amplified using PCR method and then sequenced. The data were analyzed in CLUSTAL W and PAUP4.0 software package. RESULTS\ The matK s
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