黄花倒水莲中TENUIFILIN的毛细管电泳分析

Determination of Tenuifilin in Polygala fallax Hemsl by MECC

摘要: 目的建立了毛细管电泳测定黄花倒水莲中Tenuifilin皂苷含量的方法。方法采用胶束电动毛细管电泳（40cm×75μm未涂层石英毛细管）对不同来源得黄花倒水莲提取物中Tenuifilin香苷的含量进行了测定,电泳分离条件:28mmol/L硼砂缓冲液,50mmol/LSDS,pH8.9,分离电压15kv,检测波长214um。结果在10min内可以完成样品中皂苷的含量测定,线性范围1～10μg/ml,各产地黄花倒水莲中 Tenuifilin的含量在1.02～3.41μg/g之间。结论该方法可用于黄花倒水莲的质量控制。
- 黄花倒水莲 /
- TENUIFILIN /
- 毛细管电泳 /
- 皂苷 /
- 含量测定 /
- 质量控制
Abstract: AIM A MECC (micellar electrokinetic capidary ehromatugraphy) method was established to optimize the separation of saponin in Chinese traditional medicinal plant Putt/gala falias Hemsl, and then to determine it in crude drug from different places. METHODS
- Polygala fallnx Hemsl /
- teneuifolin /
- Capillary electrophoresis