人脑胶质瘤细胞对诱导分化药物敏感性的研究

基金项目: 国家自然科学基金资助项目（39870826）

The Sensibility of Human Glioma Cell to Seven Differentiation Inducing Agents.

摘要: 目的优选人脑胶质瘤细胞诱导分化剂。方法应用MTT法、形态学、GFAP免疫荧光等方法鉴定环己亚甲基二乙酰胺、二甲基甲酰胺、丁酸钠、二丁酰环AMP、苯乙酸钠、苯丁酸钠、亚砷酸钠等7种诱导剂对人脑胶质瘤细胞系SHG－44的诱导分化效果。结果亚砷酸钠、苯乙酸钠抑制肿瘤细胞增殖效果较差，苯丁酸钠、二丁酰环AMP、丁酸钠在1－2mM即可明显诱导人脑胶质瘤细胞分化，表现为增殖抑制，胸突增长，GFAP表达升高现象。结论人脑胶质瘤细胞对诱导分化剂苯丁酸钠、二丁酰环AMP和丁酸钠较为敏感，值得深入研究。
- 药物敏感性 /
- 胶质瘤 /
- 诱导 /
- 细胞分化 /
- 苯丁酸钠 /
- 二丁酰环AMP
Abstract: AIM To study the sensibility of human glioma cells to 7 differentiation inducing agents, and screen out those most effective for glioma therapy. METHODS Human glioma cell line SHG- 44 is induced to differentiation with Hexamethelene bisacidamide (HMBA) ,
- Glioma /
- Induced /
- Cell differentiation /
- Sodium phenylbutyrate /
- Dibutyric cycle AMP