洛美利嗪对大鼠脑微血管内皮细胞上P-糖蛋白功能的影响

Effect of Lomerizine on the Activity of P-glycoprotein in Primary Cultured Rat Brain Microvessel Endothelial Cells

摘要: 目的：研究洛美利嗪对大鼠脑微血管内皮细胞上P-糖蛋白功能的影响。方法：使用荧光分光光度计和流式细胞术分析大鼠脑微血管内皮细胞内P-糖蛋白底物-罗丹明123的荧光强度。结果：环孢素A和洛美利嗪与大鼠脑微血管内皮细胞孵育后能明显提高胞内罗丹明123的荧光强度。人脐静脉内皮细胞内荧光强度则不受环孢素A和洛美利嗪的影响。结论：洛美利嗪能显著地抑制大鼠脑微血管内皮细胞上P-糖蛋白的活性，提高P-糖蛋白底物的胞内浓度。

Abstract: AIM:To study the effect of lomerizine on the activity of P-glycorprotein(P-gp) in primary cultured rat brain microvessel endothelial cells. METHOD: Fluorescence of Rhodamine123(Rh123) in RBMECs was measured by fluorescence spectrophotometry and flow cytom