西红花酸对心肌细胞氧化应激性损伤的作用

基金项目: 江苏省自然科学基金资助项目 (编号BJ19812 4)~~

Effects of Crocetin on the Myocardial Cell Damages Due to Oxidative Stress

摘要: 目的：探讨西红花酸对心肌细胞氧化应激性损伤的效应。方法：采用过氧化氢(H2O2)诱导法制备大鼠培养心肌细胞氧化应激损伤模型，通过观察培养心肌细胞的形态，检测细胞培养液中乳酸脱氢酶(LDH)活性、细胞内丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性及培养细胞台盼蓝摄取率，反映西红花酸对该模型的影响。结果：在本实验使用浓度范围内，H2O2损伤培养心肌细胞的作用呈浓度依赖性地增大；西红花酸则能减少该氧化应激细胞的形态学改变、降低培养液中LDH活性和胞内MDA含量、增加胞内SOD活性及减少细胞死亡数量。结论：西红花酸对H2O2，造成的培养心肌细胞氧化应激性损伤具有明显的保护作用。
- 西红花酸 /
- 心肌细胞 /
- 氧化应激 /
- 活性氧
Abstract: AIM: To observe the effect of crocetin on the damages induced by oxidative stress in myocardial cells. METHOD: Oxidative damage cell model was induced by H_2O_2. The morphological changes of the cells were observed, and LDH activities in the medium, intr
- Crocetin /
- Cardiomyocytes /
- Oxidative stress /
- Reactive oxygen species