五种石斛的指纹图谱研究
Studies on the HPLC/UV/MS Fingerprints of Five Kinds of Dendrobium(Shi-Hu)
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摘要: 目的:建立密花、马鞭、短棒、鼓槌和金钗等五种石斛的色谱指纹图谱。方法:采用HPLC/UV/MS技术对五种石斛的色谱指纹图谱进行了研究。结果:建立了五种石斛的HPLC/UV指纹图谱,并对其中三种石斛HPLC/MS指纹图谱中的色谱峰进行了解析,共对15个谱峰进行了化学成分的初步归属。结论:所建立的方法可用于石斛指纹图谱的测定,并为其全面质量控制及化学成分研究提供参考。Abstract: AIM: To investigate the chromatographic fi ng erprints of five kinds of Dendrobium(Shi-Hu).METHOD:Separ atio ns were performed on Agilent ZORBAX SB-C 18 and Shmadzu Shim-pack VP-OD S C 18 an alytical columns with the mobile phase consisting of a