重组人血管内皮生长因子165在Pichia酵母中的表达

Pichia Expression of Human VEGF165

摘要: 目的：采用基因工程方法构建基因工程菌制备人VEGF165蛋白。方法：自人肿瘤组织中获取人VEGF165 cDNA，构建pPIC9K酵母表达载体，采用Pichia酵母真核表达系统表达目的蛋白。结果：SDS-PAGE及Westem-blot结果显示目的蛋白已成功表达。结论：在Pichia酵母中成功表达人VEGF165。

Abstract: AIM: A recombinanted DNA Pichia pastroris was constructed to express human vascular endothelial growth factor (hVEGF 165 ) with Pichia pastroris expression system. METHOD:A re comb inant expression plasmid pPIC 9K/hVEGF165 was constructed with pPIC9