黄花倒水莲中Reinioside C含量测定及指纹图谱分析
Assay of Reinioside C in Polygala fallax Hemsl.and Fingerprint of Polygala Total Saponins
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摘要: 目的:建立黄花倒水莲中Reinioside C的含量测定方法,并对其总皂苷部位的指纹图谱进行初步研究.方法:采用RP-HPLC-ELSD法,用C18色谱柱,以乙腈-0.1%冰乙酸水溶液梯度洗脱 .结果:两点校正,自然对数换算可弥补ELSD线性应答差的不足,方法学验证此法可获得准确测定结果.结论:HPLC-ELSD法测定Reinioside C的含量及药材总皂苷部位的指纹图谱可用于黄花倒水莲的质量控制.Abstract: AIM:To establish a method for determining the contents of Reinioside C in Polygala fallax Hemsl. and to study the fingerprint of Polygala fallax Hemsl. METHOD:RP-HPLC analysis is carried out through C 18 column,using acetontrile-water as mobile phas
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