盐酸左氧氟沙星脂质体的包封率和体外释放研究

Study on Encapsulation Efficiency and In vitro Release Profile of Levofloxacin Hydrochloride Liposome

摘要: 目的:研究盐酸左氧氟沙星脂质体的包封率测定方法和影响因素,初步考察其体外释放规律.方法:用硫酸铵梯度法制备了盐酸左氧氟沙星脂质体;用凝胶过滤色谱法测定脂质体的包封率,并研究各种因素对包封率的影响;用溶出度第三法考察了脂质体的释放规律.结果:制得脂质体的包封率约为80%,脂质体的体外释放规律符合一级动力学过程.结论:体系pH值和硫酸铵浓度对包封率影响最大,制得脂质体的体外释放具有缓释特点.

Abstract: AIM:To build a determination method of encapsulation efficiency(EE)for levofloxacin liposome,to investigate the factors affecting EE,and to study the release profile in vitro.METHOD:Levofloxacin liposome was prepared by ammonium sulfate gradient method.Th