多种色谱-波谱联用技术分析栀子厚朴汤中特征成分及其配伍前后的溶出变化
Analysis of the characteristic ingredients in Zhi-Zi-Hou-Pu decoction and dissolution of these compounds in different compatibility using various hyphenated chromatographic-spectrum techniques
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摘要: 目的 ： 采用多种色谱-波谱联用技术，鉴别并归属栀子厚朴汤中的特征成分；分析主要特征成分在配伍前后的溶出变化。 方法 ： 采用高效液相色谱-紫外检测技术(LC-UV)、高效液相色谱-荧光检测技术(LC-FD)、高效液相色谱-二极管阵列检测-串联质谱联用技术(LC-PDA-MS/MS)，分析栀子厚朴汤及方药不同配伍组。色谱系统：Lichrospher C18色谱柱，甲醇-0.1%乙酸梯度洗脱。 结果 ： 从栀子厚朴汤中分离并鉴别了7个特征成分；不同配伍对6种特征成分的溶出有一定的影响。 结论 ： 各种色谱-波谱联用技术是研究中药方剂物质基础的有效手段；中药复方配伍的化学基础并非单味药化学基础的简单加合。Abstract: Aim ：To identify the characteristic ingredients in Zhi-Zi-Hou-Pu decoction (ZZHPD) by utilizing various hyphenated chromatographic-spectrum techniques and investigate the effects of the compatibility on the dissolution of the chemical components. Methods ：After separation on a Lichrospher C18 column using methanol-0.1% acetic acid gradient elution，LC-UV，LC-FD and LC-PDA-MS/MS analyses were performed for ZZHPD and the water extracts of different mixtures of crude drugs，respectively. Results ：Seven ingredients were separated and identified. The dissolution profiles of six investigated components were affected by the different composition of the crude drugs. Conclusion ：It is an effective method to study the material foundation of traditional Chinese medicines (TCMs) by hyphenated chromatographic-spectrum techniques.Some compounds in the TCMs are different form those in the raw medicinal materials.
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