香蕉皮中3-甲基-7，8-二羟基异色满酮-4的含量测定
Determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum
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摘要: 目的 ： 建立香蕉皮中3-甲基-7，8-二羟基异色满酮-4的HPLC含量测定方法。 方法 ： 色谱柱为岛津ODS-C18柱 (250 mm×4.6 mm，5 μm)，流动相：乙腈-水-磷酸(9∶91∶0.1)，检测波长283 nm，流速1 mL/min，柱温35 ℃。 结果 ： 不同批次香蕉皮原药材中3-甲基-7，8-二羟基异色满酮-4的含量为0.029 5%～0.036 7%。检测线性范围1.09～13.08 μg，平均加样回收率100.0%，RSD为0.64%(n=9)。 结论 ： 该法可作为衡量香蕉皮质量的依据。
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关键词:
- 香蕉皮 /
- 3-甲基-7，8-二羟基异色满酮-4 /
- 含量测定 /
- 高效液相色谱法
Abstract: Aim ：To establish an HPLC method for the determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum. Methods ：A column of Shimadzu ODS-C18 column(250 mm×4.6 mm，5 μm)， and a mobile phase of acetonitrile-water-phosphoric acid(9∶91∶0.1) were adopted.A detect wavelength of 283 nm，a flow rate of 1 mL/min，and a column temperature of 35 °C were set. Results ：The content of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum was 0.029 5%-0.036 7%.Calibration curve was linear over the range 1.09-13.08 μg.The average recovery was 100.0%.The assay variability value was 0.64 %(n=9). Conclusion ：The method can be used for the quality control of Musa sapientum. -
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