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香蕉皮中3-甲基-7,8-二羟基异色满酮-4的含量测定

Determination of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum

  • 摘要: 目的 : 建立香蕉皮中3-甲基-7,8-二羟基异色满酮-4的HPLC含量测定方法。 方法 : 色谱柱为岛津ODS-C18柱 (250 mm×4.6 mm,5 μm),流动相:乙腈-水-磷酸(9∶91∶0.1),检测波长283 nm,流速1 mL/min,柱温35 ℃。 结果 : 不同批次香蕉皮原药材中3-甲基-7,8-二羟基异色满酮-4的含量为0.029 5%~0.036 7%。检测线性范围1.09~13.08 μg,平均加样回收率100.0%,RSD为0.64%(n=9)。 结论 : 该法可作为衡量香蕉皮质量的依据。

     

    Abstract: Aim :To establish an HPLC method for the determination of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum. Methods :A column of Shimadzu ODS-C18 column(250 mm×4.6 mm,5 μm), and a mobile phase of acetonitrile-water-phosphoric acid(9∶91∶0.1) were adopted.A detect wavelength of 283 nm,a flow rate of 1 mL/min,and a column temperature of 35 °C were set. Results :The content of 3-methyl-7,8-dihydroxy-isochroman-4-one in the peel of Musa sapientum was 0.029 5%-0.036 7%.Calibration curve was linear over the range 1.09-13.08 μg.The average recovery was 100.0%.The assay variability value was 0.64 %(n=9). Conclusion :The method can be used for the quality control of Musa sapientum.

     

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