冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用

王莹; 尹鸿萍; 陈涛; 王旻

冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用

Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis

摘要

摘要: 目的：对麦角菌科虫草属冬虫夏草发酵菌丝体中分离得到的酸性多糖(CAPS)结构进行初步鉴定，并探讨其对顺铂诱导的肾细胞损伤的保护作用。方法：分离纯化得到CAPS，对其进行纯度及相对分子质量测定；通过紫外光谱、红外光谱、单糖组成及甲基化试验进行结构鉴定；结合血清药理学方法制备CAPS含药血清，用顺铂诱导的狗肾小管上皮细胞(MDCK)建立细胞模型。结果：纯度99%的CAPS相对分子质量为2.7×10⁴，由甘露糖、葡萄糖、半乳糖及少量木糖、阿拉伯糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸组成。对顺铂诱发的肾细胞损伤的保护作用在含药血清大于2.5%时，与顺铂模型组相比，有显著性差异(P＜0.05)；大于5%时，与模型组相比，有极显著性差异(P＜0.01)。结论： CAPS具有显著的体外保护肾细胞损伤的作用。

Abstract: Aim ：To elucidate the structure of an acidic polysaccharide from Cordyceps sinensis and to study its protective effect on renal cell injury by cisplatin. Methods ：After Cordyceps sinensis acidic polysaccharide(CAPS) was isolated and purified，the purity and molecule weight were detected by high performance gel permeation chromatography(HPGPC).Based on the UV spectrum，FT-IR spectrum，monosaccharide composition and GC-MS，the structures were elucidated.Cisplatin-induced injury of Madin-Darby canine kidney（MDCK） cell line was protected by the blood serum with CAPS. Results ：The purity of CAPS，with a molecule weight of 2.7×10⁴，was about 99%.It consisted mainly of mannose，glucose and galactose.When the serum with CAPS reached 2.5%，it showed difference compared with the cisplatin group(P＜0.05) on the protective effect of renal cell injury.It showed significant difference(P＜0.01) when the serum with CAPS exceeded 5%. Conclusion ：CAPS has a protective effect on the renal cell injury in vitro.

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