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冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用

王莹, 尹鸿萍, 陈涛, 王旻

王莹, 尹鸿萍, 陈涛, 王旻. 冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用[J]. 中国药科大学学报, 2009, 40(6): 559-564.
引用本文: 王莹, 尹鸿萍, 陈涛, 王旻. 冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用[J]. 中国药科大学学报, 2009, 40(6): 559-564.
WANG Ying, YIN Hong-ping, CHEN Tao, WANG Min. Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis[J]. Journal of China Pharmaceutical University, 2009, 40(6): 559-564.
Citation: WANG Ying, YIN Hong-ping, CHEN Tao, WANG Min. Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis[J]. Journal of China Pharmaceutical University, 2009, 40(6): 559-564.

冬虫夏草酸性多糖的结构初步鉴定及对肾细胞损伤的保护作用

基金项目: 国家十一五“重大新药创制”科技重大专项基金资助项目(No.2009ZX09102-111)

Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis

  • 摘要: 目的 : 对麦角菌科虫草属冬虫夏草发酵菌丝体中分离得到的酸性多糖(CAPS)结构进行初步鉴定,并探讨其对顺铂诱导的肾细胞损伤的保护作用。 方法 : 分离纯化得到CAPS,对其进行纯度及相对分子质量测定;通过紫外光谱、红外光谱、单糖组成及甲基化试验进行结构鉴定;结合血清药理学方法制备CAPS含药血清,用顺铂诱导的狗肾小管上皮细胞(MDCK)建立细胞模型。 结果 : 纯度99%的CAPS相对分子质量为2.7×104,由甘露糖、葡萄糖、半乳糖及少量木糖、阿拉伯糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸组成。对顺铂诱发的肾细胞损伤的保护作用在含药血清大于2.5%时,与顺铂模型组相比,有显著性差异(P<0.05);大于5%时,与模型组相比,有极显著性差异(P<0.01)。 结论 : CAPS具有显著的体外保护肾细胞损伤的作用。
    Abstract: Aim :To elucidate the structure of an acidic polysaccharide from Cordyceps sinensis and to study its protective effect on renal cell injury by cisplatin. Methods :After Cordyceps sinensis acidic polysaccharide(CAPS) was isolated and purified,the purity and molecule weight were detected by high performance gel permeation chromatography(HPGPC).Based on the UV spectrum,FT-IR spectrum,monosaccharide composition and GC-MS,the structures were elucidated.Cisplatin-induced injury of Madin-Darby canine kidney(MDCK) cell line was protected by the blood serum with CAPS. Results :The purity of CAPS,with a molecule weight of 2.7×104,was about 99%.It consisted mainly of mannose,glucose and galactose.When the serum with CAPS reached 2.5%,it showed difference compared with the cisplatin group(P<0.05) on the protective effect of renal cell injury.It showed significant difference(P<0.01) when the serum with CAPS exceeded 5%. Conclusion :CAPS has a protective effect on the renal cell injury in vitro.
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  • 刊出日期:  2009-12-24

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