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丹参酮IIA对乳腺癌细胞MDA-MB-435迁移及肿瘤血管生成的影响(英文)

Anti-migration and anti-angiogenic effects of tanshinone IIA on breast cancer cell MDA-MB-435

  • 摘要: 目的 : 研究丹参酮IIA抑制迁移以及新生血管形成的作用机制。 方法 : 通过MTT,2-D/3-D迁移,血管生成,PCR,CAM等方法,评价丹参酮IIA抑制肿瘤细胞增殖,迁移以及新生血管的药理学活性,探讨其可能的作用机制。 结果 : 给药24 h后,丹参酮IIA能够抑制乳腺癌细胞(MDA-MB-435)增殖(IC50=21 nmol/mL),并且当浓度分别大于5 nmol/mL和6 nmol/mL时,对癌细胞的2-D和3-D迁移具有很好的抑制作用。但其对新生牛主动脉内皮细胞(NCAEC)增殖几乎没有影响(IC50=124 nmol/mL)。共培养法tube formation实验发现,丹参酮IIA抑制新生血管形成主要作用其第2阶段,即内皮细胞的分化阶段。在基因转录水平上,丹参酮IIA对肿瘤增殖、迁移以及血管生成相关基因VEGF,c-Myc及HIF-1α表达具有良好的抑制作用。CAM实验表明,丹参酮IIA对新生血管生成也有良好的抑制作用。 结论 : 丹参酮IIA具有很好的抑制肿瘤增殖,迁移及血管生成作用。其可能的作用机制是通过抑制VEGF、c-Myc及HIF-1α的表达,从而产生药理学活性。

     

    Abstract: Aim :To investigate the mechanism of anti-proliferation,anti-migration and anti-angiogenic effects of tanshinone IIA. Methods :In this study,MTT,2-D/3-D migration,tube formation,PCR,chick embryo chorioallantoic membrane (CAM) were used to evaluate the anti-proliferation,anti-migration and anti-angiogenic effects of tanshinone IIA. Results :Tanshinone IIA significantly inhibited the proliferation of human breast cancer line MDA-MB-435 with an IC50 of 21 nmol/mL.And it is showed that breast cancer cells migration was effectively prevented by tanshinone IIA at 5 and 6 nmol/mL in a wound healing assay and a transwell migration assay.In addition,tanshinone IIA inhibited the tube formation of newborn cattle aortic endothelial cells(NCAECs) after 2 h co-incubation with MDA-MB-435.These effects were not due to the inhibition of NCAECs proliferation;because tanshinone IIA non-selectively inhibited NCAECs growth with an IC50 of 124 nmol/mL.Tanshinone IIA showed dose-dependent inhibitory effects on mRNA expression of VEGF and two transcription factors (HIF-1α/c-Myc). Tashinone IIA was also found to inhibit angiogenic in vivo in the CAM assay. Conclusion :These results suggest that tanshinone IIA may exert its anti-proliferation,anti-migration and anti-angiogenic effects through down-regulating two transcription factors and VEGF.These novel anti-proliferations,anti-migrations,anti-angiogenic activities of tanshinone IIA are likely to contribute to its cancer chemopreventive and therapeutic potential,especially in the treatment of breast cancer.

     

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