内标校正法在中药栀子多成分同时定量分析中的应用

基金项目: 国家“重大新药创制”科技重大专项资助项目（No.2009ZX09308-005）；中医药行业科研专项资助项目(No.200707009)；教育部新世纪优秀人才支持计划资助项目(No.NCET-06-0497)

Application of internal standard correction method in the simultaneous determination of multi-components of Fructus Gardeniae

摘要: 目的： 建立一种方便实用的中药栀子的质量评价方法。 方法： 建立西红花苷-1与其他西红花酸类成分的内标校正因子(f_km)并考察其系统适应性，测定西红花苷-1的含量，用f_km计算其它西红花酸类成分的含量。 结果： 内标校正法计算与实测分析结果无显著差异。 结论： 内标校正法在中药栀子定量分析中具可行性。
- 内标校正法 /
- 栀子 /
- 定量分析
Abstract: Aim： To establish a simple and convenient quality evaluation method for Fructus Gardeniae(FG). Methods： The internal standard correction factors (f_km) of four crocetin derivatives to crocin-1 were established and the system suitability was tested.The content of crocin-1 was determined by external standard method，the contents of other crocetin derivatives were calculated by the content of crocin-1 and their f_km. Results： There was no significant difference between the test results obtained by the methods of “theoretical calculation by f_km” and “routire determination technique”. Conclusion： The internal standard correction method(the method of “theoretical calculation by f_km”) is practical and feasible in the quantitative analysis of Fructus Gardeniae.
- internal standard correction method /
- Fructus Gardeniae /
- quantitative analysis