Abstract: Aim： To establish a simple and convenient quality evaluation method for Fructus Gardeniae(FG). Methods： The internal standard correction factors (f_km) of four crocetin derivatives to crocin-1 were established and the system suitability was tested.The content of crocin-1 was determined by external standard method，the contents of other crocetin derivatives were calculated by the content of crocin-1 and their f_km. Results： There was no significant difference between the test results obtained by the methods of “theoretical calculation by f_km” and “routire determination technique”. Conclusion： The internal standard correction method(the method of “theoretical calculation by f_km”) is practical and feasible in the quantitative analysis of Fructus Gardeniae.