Abstract:
Abstract Aim: To explore the relationship between structure and immunological activity of marine polysaccharide YCP,the physicochemical property and immunological activity of the YCP-derived fragment were studied.
Methods: YCP was hydrolyzed by α-amylase from human saliva.The hydrolysate was purified to obtain an polysaccharide fragment by gel filtration chromatography.The physicochemical properties of this YCP-derived fragment was characterized by HPLC,FT-IR and TLC.In addition,changes of phagocytic activity,production of reactive nitrogen and macrophage binding were investigated.
Results: The relative molecular weight of YCP-derived fragment was approximately 6.6×10
3.The monosaccharide composition and FT-IR of the YCP-derived fragment were identical to YCP.No significant effect of the YCP-derived fragment on NO production and murine macrophage phagocyte were observed.And this fragment was not able to compete the binding between YCP and macrophages.
Conclusion: The remarkable decrease of immunological activity of YCP-derived fragments degraded by α-amylase of human saliva suggests that the complete structure and high molecule weight of YCP are essential for its immuno-modulatory activity.