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新型穿心莲内酯衍生物的合成及抗肿瘤作用

Synthesis and antitumor effect of novel andrographolide derivatives

  • 摘要: 以穿心莲内酯为先导物,合成了一系列结构为12-N-取代-14-脱氧穿心莲内酯的衍生物。初步评价了这些衍生物的体外抗肿瘤活性,筛选出活性显著高于穿心莲内酯的化合物 4d 。对于高活性化合物 4d ,以人肝癌HepG2细胞为体外模型,小鼠H22和S180皮下移植性肿瘤为体内模型,进一步观察其药效,发现化合物 4d 在体外和体内均具有显著的抗肿瘤作用。通过Annexin V/PI双染分析检测出加药后HepG2细胞的凋亡率明显增加。深入的机制研究表明,化合物 4d 能够使HepG2 细胞中p53和Bax表达增加,同时使Bcl-2表达减少。化合物 4d 具有显著的体内外抗肿瘤作用,其机制可能与激活p53依赖性凋亡诱导途径有关,值得进一步研究。

     

    Abstract: A series of andrographolide derivatives with the structure of 12-N-substituted-14-deoxyandrographolide were synthesized from the parent compound andrographolide.Their antitumor activities were preliminarily evaluated on various cancer cell lines and compound 4d stood out due to its potent growth inhibitory effect in comparison with andrographolide.Compound 4d also demonstrated significant antitumor effect on human hepatoma HepG2 cells in vitro and on sarcoma 180 (S180) and hepatoma 22 (H22)-bearing mice in vivo.Then,the apoptosis induced by compound 4d in HepG2 cells was detected by Annexin V/PI double staining assay.Further mechanic study showed that the expression of p53 and Bax was significantly elevated and that of Bcl-2 was down-regulated in 4d -treated HepG2 cells.Collectively,these data suggested that compound 4d had remarkable antitumor effect both in vitro and in vivo and could effectively induce apoptosis via a p53-dependent pathway in HepG2 cells,thus deserving further investigation.

     

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