酶联免疫法测定知母中菝葜皂苷元的含量

基金项目: 教育部科学技术研究重点项目资助(No.08071)；江苏省普通高校研究生科研创新计划资助项目(No.CX08B_192Z)

An enzyme-linked immunosorbent assay for the determination of sarsasapogenin in Rhizoma Anemarrhenae

摘要: 应用菝葜皂苷元多克隆抗体建立检测知母中菝葜皂苷元含量的酶联免疫分析方法，结果显示其检测线性范围为20～1 311 ng/mL，最低检出浓度为20 ng/mL。样品加样回收率为92.2%～97.1%，日内日间RSD均小于14.3%。以所建立的方法检测5个产地知母中菝葜皂苷元的含量，检测结果与HPLC-ELSD分析结果一致。本实验建立的菝葜皂苷元免疫分析方法具有简便、灵敏、快速的特点，可简化中药复杂体系中单一成分的含量测定。
- 酶联免疫法 /
- 多克隆抗体 /
- 菝葜皂苷元 /
- 知母
Abstract: Using polyclonal antibody against sarsasapogenin，an enzyme-linked immunosorbent assay (ELISA) for quantitative analysis of sarsasapogenin in Rhizoma Anemarrhenae was developed.The linear detection range of sarsasapogenin was 20-1 311 ng/mL.The lowest limit of detection(LOD) of the assay was 20 ng/mL，and the recovery rate ranged from 92.2% to 97.1%.The relative standard deviation for intra- and inter- assays were less than 14.3%.To validate the immunoassay，the sarsasapogenin content of Rhizoma Anemarrhenae from 5 different habitats were analyzed by the immunoassay and HPLC-ELSD.The results obtained by the two methods were consistent.This immunoassay is simple，sensitive and rapid，and could simplify the determination of the single components in multi-component system.
- ELISA /
- polyclonal antibody /
- sarsasapogenin /
- Rhizoma Anemarrhenae