Abstract: The purpose of this study was to exploit the potential of Primer Premier 5.0 and RNA structure 4.6 in the design and selection of effective 10-23 deoxyribozyme (DRz) targeting resistance gene mecR1.Five designed and synthesized anti-mecR1 10-23 DRz sequences were introduced into the MRSA strain by electro transformation in vivo.Transcription of mecR1 was analyzed by real-time quantitative PCR.The inhibitory effects of DRzs on the bacterial growth were evaluated based on the plate cloning formation.It was found that the five anti-mecR1 10-23 DRz sequences significantly lowered the transcription of mecR1 and inhibited the growth of clinical drug-resistant MRSA080309，with DRz6 having the most significant inhibitory effect.Therefore，the combination of the two computer softwares is an economical，practical and effective approach in the design of anti-mecR1 DRz，and could greatly reduce the screening time of antisense drugs.