款冬花乙酸乙酯部位对炎症因子释放的影响
Effect of the ethyl acetate extract of Farfarae Flos on inflammatory factors
-
摘要: 研究款冬花乙酸乙酯部位(FF-EtOAc)对炎症因子释放的影响及作用机制。采用脂多糖（LPS）诱导RAW264.7细胞活化模型,分别用Griess法、ELISA、Western-blot法检测NO的释放、TNF-α和IL-6的含量、磷酸化NF-κB P-65的表达。结果显示在LPS刺激下,RAW264.7细胞中上述检测指标均显著提高。而FF-EtOAc和阳性对照组水杨酸钠均能抑制LPS诱导的活化RAW264.7细胞释放NO,能浓度依赖性抑制TNF-α和IL-6产生并且能显著抑制NF-κB家族P-65的磷酸化。研究证实FF-EtOAc能够显著抑制炎性因子的释放,缓解炎症的病理过程;据此推测可能与NF-κB激活的调节有关。Abstract: The effect and mechanism of the ethyl acetate extract of Farfarae Flos (FF-EtOAc) on inflammatory factors in LPS-stimulated RAW 264.7 model was studied in this paper.The levels of NO,TNF-α,and IL-6 were tested by Griess assay and ELISA,and NF-κB binding protein was tested by Western blot.Results showed that higher levels of NO,IL-6 and TNF-α were released,and the level of phosphorylation of NF-κB P-65 was higher than normal group;both FF-EtOAc and sodium salicylate had inhibitory effect on NO,IL-6 and TNF-α production in LPS-stimulated RAW 264.7 cells.Western blot results demonstrated that FF-EtOAc can significantly inhibit LPS- induced phosphorylation of NF-κB P-65 in RAW 264.7.We inferred that the mechanisms of FF-EtOAc anti- inflammatory effect in LPS-stimulated RAW 264.7 may be through inhibiting of NF-κB P-65 phosphorylation to reduce the production of NO,TNF-α and IL-6.
-
Keywords:
- Farfarae Flos /
- inflammatory factors /
- RAW264.7 /
- TNF-α /
- IL-6 /
- NF-κB
-
计量
- 文章访问数: 1468
- HTML全文浏览量: 0
- PDF下载量: 2028
下载:
