乌索酸通过抑制A549细胞中ERK的激活而抑制COX-2的表达(英文)

Ursolic acid downregulates COX-2 expression by suppressing the activation of ERK in A549 cells

摘要: 研究乌索酸对A549细胞的抗肿瘤作用及其机制。首先，通过MTT方法检测乌索酸对A549细胞增殖的影响；然后，用Western blot方法检测乌索酸对COX-2表达水平及MAPKs的作用。结果表明，乌索酸能够抑制A549细胞的增殖，并能抑制COX-2的表达及ERK的激活，ERK特异性抑制剂PD98059能够与乌索酸协调抑制COX-2的表达。乌索酸能够抑制LPS刺激后A549细胞中COX-2的表达，其可能的作用机制是通过抑制ERK的激活。

Abstract: The antitumer effect and the mechanism of action of ursolic acid in A549 cells (human non-small-cell lung cancer cells) was investigated in this paper.Firstly,MTT assay was performed to test whether ursolic acid could inhibit the growth of A549 cells.Secondly,Western blot was utilized to measure the expression level of COX-2 and the activation of MAPKs.The MTT assay revealed that ursolic acid inhibited the growth of A549 cells.The result of Western blot suggested that ursolic acid inhibited the expression of COX-2 and activation of ERK and ERK specific inhibitor PD98059 suppressed the expression of COX-2 synergistically with ursolic acid.Taken together,our data suggest that ursolic acid can suppress LPS-induced COX-2 expression in A549 cells,which could be due to the inhibition of the activation of ERK.