Abstract: To investigate the antitumor and anti-angiogenesis effects of vascular endothelial growth factor 6a (VEGF6a),VEGF6a and green fluorescent protein (GFP) gene were linked by GGGS segment and cloned into pET28a vector by PCR.GFP-VEGF6a (GFP6a) fusion polypeptides were expressed in E.coli and purified by heparin sepharose chromatograph.ICR mice bearing H22 hepatoma carcinoma cells was treated with subcutaneous injection of GFP6a and GFP.Compared with the negative control GFP,GFP6a (200mg/kg,20 mg/kg) significantly inhibited the growth of the peripheral blood vessels and H22 cells in vivo,with the inhibition ratios of 70.9% and 56.1% (P<0.01),respectively.VEGF6a has the potency in the application of anticancer clinical therapy.