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LC-MS/MS法测定人血浆中奈比洛尔浓度及其在中国人体内的药代动力学

Determination of nebivolol in human plasma by LC-MS/MS and study of its pharmacokinetics on the Chinese

  • 摘要: 本文建立了一种快速测定人血浆中奈比洛尔血药浓度的LC-MS/MS法,并研究其在中国健康人体内的药代动力学行为。以氨氯地平作为内标,采用C18反相柱(150 mm×2.0 mm,4.6 μm),柱温35 ℃。流动相为乙腈-水(含0.05%甲酸)(45∶55),流速0.2 mL/min;电喷雾离子化(ESI),正离子扫描,选择性反应监测(SRM)药和内标分别为:奈比洛尔m/z 406.2→151.0;氨氯地平m/z 409.0→238.2。在本文建立的方法下,奈比洛尔在0.025~25 ng/mL呈良好的线性关系,r=0.998 6,最低检测浓度为0.008 ng/mL,低、中、高浓度下的回收率、日内及日间精密度均符合方法学要求。健康受试者口服5 mg奈比洛尔片后的t1/2,AUC0-tcmax,MRT分别为:(14.4±5.5) h,(7.35±2.48)ng?h/mL,(1.05±0.35) ng/mL,(16.5±5.3) h。结果表明:该方法专属性强,适用于奈比洛尔血样的定量分析。

     

    Abstract: A simple,sensitive and rapid liquid chromatographic-electro-spray ionization tandem mass spectrometric method was developed for the quantification of nebivolol in human plasma.Amlodipine was used as the internal standard(IS).Separation was performed using a Shimadzu C18column (150 mm×2.0 mm,4.6μm) maintained at 35℃ and the mobile phase consisting of a mixture of acetonitrile and water (containing 0.05% formic acid) (45∶55) was delivered at a flow rate of 0.2 mL/min;The analytes were analyzed by electro-spray ionization (ESI) in the selected reaction monitoring mode.The precursor to product ion transitions ofm/z 406.2-151.0 and m/z 409.0-238.2 were used to measure nebivolol and the IS,respectively.The linearity ranged from 0.025 to 25 ng/mL( r = 0.998 6);and the limit of detection of nebivolol in human plasma was 0.008 ng/mL.The recovery,intra- and inter-assay precisions met the requirements of bioanalytical method.Thet1/2,AUC0-t,cmax,MRT of nebivolol in healthy volunteers were (14.4±5.5)h,(7.35±2.48) ng?h/mL,(1.05±0.35) ng/mL,and (16.5±5.3)h for a single oral dose of 5 mg nebivolol,respectively.The method is selective and suitable for the quantification of nebivolol in human plasma.

     

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