贻贝水解物抗肿瘤活性肽的分离纯化
Isolation and purification of an anticancer activity peptide from protein hydrolysate of Mytilus coruscus
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摘要: 采用超滤法、DEAE-sepharose离子交换法、Sephadex G-25凝胶层析法和反相高效液相色谱法从贻贝水解物中分离得到一个具有抗肿瘤活性的多肽。以CCK-8法作为各分离阶段抗肿瘤活性的筛选方法。最终获得一个高纯度多肽，经检测该肽的氨基酸序列为Asp-Leu-Tyr。Abstract: In this studies, an anticancer peptide was isolated from the enzymatic hydrolysate of Mytilus coruscus. CCK-8 assay was used as guide in activity screening through the isolation process of membrane filtration, DEAE-sepharose chromatography, Sephadex G-25 exclusive chromatography and RP-HPLC. An anticancer peptide was obtained and identified as Asp-Leu-Tyr.
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