高密度发酵解脂假丝酵母生产RNA的工艺研究
Fermentaton process for preparation of RNA from Candida tropicalis
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摘要: 为进一步优化RNA生产工艺，通过对解脂假丝酵母发酵培养，研究了pH、溶氧、还原糖浓度、高密度补料分批发酵和高密度连续发酵等因素对RNA产量及还原糖转化成菌体的转化率（g-DCW/g-reducing sugar）的影响。实验结果表明，最佳培养条件为pH 4，溶氧30%。在分批发酵中，培养基糖浓度为40 g/L时，最高DCW (dry cell weight)可达19.5 g/L，培养时间为8 h，RNA最高含量达到14.3%（g-RNA/g-DCW），转化率为48.2%。在最佳pH和溶氧条件下进行pH-stat补糖分批发酵，控制罐内培养基糖浓度始终维持在15 g/L以下，最高核酸含量达到14.2%，转化率达到43.5%，最高菌体浓度35.6 g/L，比40 g/L糖浓度条件下分批发酵时的菌体浓度提高了75%。在此基础上进行高密度连续发酵，最终结果与高密度分批发酵相似。首次实现了高密度发酵解脂假丝酵母制备RNA。Abstract: In this study,effects of the pH,dissolved oxygen (DO) and reducing sugar concentration,high density batch fermentation and high density continuous fermentation on RNA production and reducing sugar conversion rate (g-DCW/g-Glucose) were investigated by the fermentation of Candida tropicalis.It was found that the optimal condition was of pH 4 and DO of 30%.In batch fermentation,19.5 g/L of dry cell concentration was obtained at 40 g/L reducing sugar and the total fermentation time was 8 h and the RNA lever reached 14.3%.In pH-stat fed-batch fermentation by pH and DO at optimal levels,cell concentration reached 35.6 g/L which was 75% higher than that of 19.5 g/L(40 g/L glucose) with the RNA level of 14.2% and reducing sugar conversion rate of 43.5%.The results showed that high density continuous fermentation was similar to high density batch fermentation.
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Keywords:
- RNA /
- production /
- Candida tropicalis /
- dissolved oxygen /
- fed-batch fermentation
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