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高密度发酵解脂假丝酵母生产RNA的工艺研究

林忠, 罗众球, 窦洁, 王慧, 邱蔚然, 曹静, 周长林

林忠, 罗众球, 窦洁, 王慧, 邱蔚然, 曹静, 周长林. 高密度发酵解脂假丝酵母生产RNA的工艺研究[J]. 中国药科大学学报, 2011, 42(4): 369-374.
引用本文: 林忠, 罗众球, 窦洁, 王慧, 邱蔚然, 曹静, 周长林. 高密度发酵解脂假丝酵母生产RNA的工艺研究[J]. 中国药科大学学报, 2011, 42(4): 369-374.
LIN Zhong, LUO Zhong-qiu, DOU Jie, WANG Hui, QIU Wei-ran, CAO Jing, ZHOU Chang-lin. Fermentaton process for preparation of RNA from Candida tropicalis[J]. Journal of China Pharmaceutical University, 2011, 42(4): 369-374.
Citation: LIN Zhong, LUO Zhong-qiu, DOU Jie, WANG Hui, QIU Wei-ran, CAO Jing, ZHOU Chang-lin. Fermentaton process for preparation of RNA from Candida tropicalis[J]. Journal of China Pharmaceutical University, 2011, 42(4): 369-374.

高密度发酵解脂假丝酵母生产RNA的工艺研究

基金项目: 江苏省高校科研成果产业化推进项目资助(No.JH10-12)

Fermentaton process for preparation of RNA from Candida tropicalis

  • 摘要: 为进一步优化RNA生产工艺,通过对解脂假丝酵母发酵培养,研究了pH、溶氧、还原糖浓度、高密度补料分批发酵和高密度连续发酵等因素对RNA产量及还原糖转化成菌体的转化率(g-DCW/g-reducing sugar)的影响。实验结果表明,最佳培养条件为pH 4,溶氧30%。在分批发酵中,培养基糖浓度为40 g/L时,最高DCW (dry cell weight)可达19.5 g/L,培养时间为8 h,RNA最高含量达到14.3%(g-RNA/g-DCW),转化率为48.2%。在最佳pH和溶氧条件下进行pH-stat补糖分批发酵,控制罐内培养基糖浓度始终维持在15 g/L以下,最高核酸含量达到14.2%,转化率达到43.5%,最高菌体浓度35.6 g/L,比40 g/L糖浓度条件下分批发酵时的菌体浓度提高了75%。在此基础上进行高密度连续发酵,最终结果与高密度分批发酵相似。首次实现了高密度发酵解脂假丝酵母制备RNA。
    Abstract: In this study,effects of the pH,dissolved oxygen (DO) and reducing sugar concentration,high density batch fermentation and high density continuous fermentation on RNA production and reducing sugar conversion rate (g-DCW/g-Glucose) were investigated by the fermentation of Candida tropicalis.It was found that the optimal condition was of pH 4 and DO of 30%.In batch fermentation,19.5 g/L of dry cell concentration was obtained at 40 g/L reducing sugar and the total fermentation time was 8 h and the RNA lever reached 14.3%.In pH-stat fed-batch fermentation by pH and DO at optimal levels,cell concentration reached 35.6 g/L which was 75% higher than that of 19.5 g/L(40 g/L glucose) with the RNA level of 14.2% and reducing sugar conversion rate of 43.5%.The results showed that high density continuous fermentation was similar to high density batch fermentation.
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出版历程
  • 刊出日期:  2011-08-24

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