重组鲨肝刺激物质类似物对刀豆蛋白A致小鼠急性肝损伤的保护作用
Protective effect of recombinant hepatical stimulator analogue on concanavalina A-induced acute liver injury in mice
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摘要: 为探讨重组鲨肝刺激物质类似物(r-sHSA)对刀豆蛋白A（Con A）致小鼠急性肝损伤的保护作用及其作用机制，利用尾静脉注射Con A制备小鼠急性肝损伤动物模型，以谷丙转氨酶（ALT）和谷草转氨酶（AST）活性以及组织病理变化为指标判断r-sHSA(30，60，120 μg/kg)对免疫性肝损伤的保护作用；同时，检测肝组织丙二醛、谷胱甘肽水平和总抗氧化能力以及血清中TNF-α和IFN-γ的含量，并测定肝组织匀浆中iNOS活力。结果表明，3个给药剂量的r-sHSA均可显著降低肝损伤小鼠血清转氨酶活性，明显减轻肝细胞坏死，减少炎细胞浸润，改善肝脏氧化应激水平，并下调炎症因子表达。结果提示，r-sHSA对Con A致小鼠急性肝损伤具有明显的保护作用，其机制可能与抗氧化和抗炎作用有关。Abstract: To study the effect of recombinant shark hepatical stimulator analogue (r-sHSA) on Con A-induced acute liver injury in mice,serum aminotransferase activities and histological changes of hepatocytes were measured to assess the protective effect of r-sHSA (30,60,120 μg/kg).Hepatic malondialdehyde (MDA),glutathione (GSH) and total antioxidative capacity (T-AOC) levels,serum TNF-αand IFN-γ levels,and the activity of iNOS in hepatic homogenates were measured,respectively.Within all the three treated dosages,r-sHSA exhibited significant hepatoprotective effect by decreasing mouse serum aminotransferase activities,ameliorating hepatocyte necrosis and reducing inflammatory cell infiltration.The protection mechanism might be related with the reduced hepatic oxidative stress and proinflammatory cytokine production by r-sHSA pretreatment.These results indicated that r-sHSA could protect mice from Con A-induced acute liver injury through its antioxidant and anti-inflammatory activities.
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