高级检索

猪牙皂药材的质量评价方法

Quality evaluation of Gleditsiae Fructus Abnormalis

  • 摘要: 以prosapogenin 2b为指标成分,采用HPLC法研究猪牙皂药材质量评价方法。采用汉邦Lichrospher C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.5%冰醋酸(36∶64),流速为1.0 mL/min,柱温35 ℃,检测波长205nm。结果表明,prosapogenin 2b在5.38~32.28μg/mL范围内线性关系良好,低、中、高3种浓度的平均回收率(%)分别为101.58,97.39和96.85,RSD为2.92%。本法为猪牙皂药材质量标准建立和品质评价提供了依据。

     

    Abstract: An HPLC method was developed for the quality evaluation of Gleditsiae Fructus Abnormalis based on prosapogenin 2b.HPLC separation was achieved on a Lichrospher C 18 column using acetonitrile-0.5% acetic acid (water) (36∶64) as the mobile phase with the flow rate of 1.0 mL/min and the detertion wavelength of 205nm.The linear range for prosapogenin 2b was from 5.38 to 32.28 μg/mL.The average recoveries measured at three levels were 101.58%,97.39%,96.85%,respectively.This method may provide the basis for quality control and evaluation of Gleditsiae Fructus Abnormalis.

     

/

返回文章
返回