Abstract: Nitric oxide (NO) is a ubiquitous signaling molecule,which takes an important part in various complicated physiological process,such as neurotransmission,energy metabolism,immune response,inflammatory response and tumorigenesis.Therefore,the detection of NO is of great significance.The conventional methods of NO detection are not able to determine NO level in vivoat a real-time scale and the application of these methods are frequently restricted.In this review,we focus on the development of copper (II) compound-derived NO fluorescent probes that have been comprehensively used for the determination of NO level in the cultured cells andin vivo.