Abstract: gliT is a member of gliotoxin biosynthesis gene cluster.To know its role in the biosynthesis of gliotoxin,a gliT gene was cloned from Aspergillus fumigatus strain F3.This gene is deduced to encode a putative thioredoxin reductase,which shares about 97% homology with the GliT from A.fumigatus strain Af293 and contains the conserved domains of Pyr_redox and TrxB.The expression levels of this gene showed a significant positive correlation with the gliotoxin contents in the fermentation broth of A.fumigatus strain F3 under conditions of increasing salinity,and reached their peaks under 70 g/L salinity.These results showed that the role of gliT on the biosynthesis of gliotoxin may be related to salt (or salinity) stresses.