紫杉醇高产菌发酵产物的分离、纯化和鉴定
Isolation,purification and identification of paclitaxel from the fermented extracts of fungus ST026（Altemaria alternate var.monosporus）
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摘要: 真菌ST026（Altemaria alternate var.monosporus）的发酵产物,经过常压正相硅胶柱，中压正相硅胶柱，中压反相ODS柱色谱和重结晶等4步纯化得紫杉醇（纯度99.0%），4步总收率0.003 9%（以发酵浸膏计），紫杉醇的回收率为38.5%。Abstract: Four steps,including normal silica gel chromatography,medium pressure silica gel chromatography,medium pressure ODS RP-chromatography and recrystalization,were used to isolate and purify paclitaxel from the fermented extracts of ST026 (Altemaria alternate var.monosporus).The total yield of paclitaxel was 0.003 9% with the purity of 99.0% and the recovery of of 38.5%.
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Keywords:
- fungus /
- fermentation /
- purify /
- identification /
- paclitaxel /
- alternol
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