Abstract:
The objective was to investigate the influencing factors on the siRNA delivery efficiency of hydrophobic modified hyaluronic acid with molecular weight 9 600 grafted with spermine and laurylamine cationic micelles(HAPL
9600).The transfection efficiency of HAPL
9600/siRNA was determined by flow cytometry.The influence of the molecular weight of HA,complexes forming time,the concentration of the HAPL
9600,pH,the transfection medium and the weight ratio of HAPL
9600 to siRNA on transfection were investigated.The competitive inhibition experiment was studied to investigate the
in vivo cell target ability.The results indicated that HAPL
9600 would reach the optimal transfection efficiency when the molecular weight of HA is 9 600 with 30 min of complexes forming time,15 mg/mL of concentration of the HAPL
9600,8 of the weight ratio of HAPL
9600 to siRNA at PBS(pH 6.8) transfection medium.The transfection efficiency of the HAPL
9600 was reduced by competitive binding of free HA onto the CD
44 receptors,thereby lowering the extent of cellular uptake via CD
44 receptor mediated endocytosis,which suggested its in vitro targeting ability on the tumor cells.In all,HAPL
9600 could obtain optimal,reduplicate and targeting transfection results by controlling related factors.