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LC-MS/MS法测定人血浆中8′-羟基二氢麦角隐亭浓度及其在生物等效性研究中的应用

Determination of 8′-hydroxy-dihydroergocryptine in human plasma by LC-MS/MS and its application to a bioequivalence evaluation

  • 摘要: 建立一种快速测定人血浆中8′-羟基二氢麦角隐亭 (8′-OH-DHEC)浓度的LC-MS/MS 法,以曲马多为内标,采用C18反相柱(150 mm×2.0 mm,5 μm),柱温35 ℃。流动相为甲醇-水(含0.1%甲酸),流速0.2 mL/min;电喷雾离子化(ESI),正离子扫描,选择性反应监测(SRM)8′-OH-DHEC m/z 594.3→270.0;曲马多m/z 264.1→58.3。本方法专属性强,8′-OH-DHEC在50~4 000 pg/mL 呈良好的线性关系,r =0.999 3,最低检测浓度为50 pg/mL,低、中、高浓度下的日内及日间精密度、准确度、稳定性等均符合生物样品测定要求。本方法应用于22名健康受试者口服4 mg 麦角隐亭的生物等效性研究,结果表明此方法专属性强,快速灵敏,适用于临床剂量下麦角隐亭药代动力学及生物等效性研究。

     

    Abstract: A sensitive,selective and simple liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for determining the major active metabolite 8′-hydroxy-dihydroergocryptine (8′-OH-DHEC) of α-dihydroergocryptine (DHEC) in human plasma.The analyte was extracted from plasma samples by liquid-liquid extraction,separated through a Shimadzu shim-pack VP-ODS column (150 mm×2.0 mm,5 μm) using isocratic mobile phase consisting of 0.1% formic acid- methanol (48 ∶52),and analyzed by electro-spray ionization (ESI) in the selected reaction monitoring mode.The precursor to product ion transitions of m/z594.3→270.0 and m/z 264.1→58.3 were used to measure 8′-OH-DHEC and the IS tramadol,respectively.The method was validated over a concentration range of 50-4 000 pg/mL.The intra- and inter-day precision values were less than 10% and accuracy values 90%-110%.The stability of 8′-OH-DHEC in human plasma under different storage conditions met the requirements of bioanalytical method.The established method was successfully applied to a bioequivalence study after single oral administration of 4 mg DHEC mesilate (test and reference) in 22 healthy volunteers.The 90% confidence intervals of log‐transformed ratios of the cmaxand AUC0-twere 89.68%-129.9%,106.9%-121.8%,respectively.The method is selective and suitable for the quantification of 8′-OH-DHEC in human plasma.

     

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